Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k . a 109bp fragment was amplified from the human genome dna . 2 . in 166 han samples , 19 ( 11 . 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp 用紅細(xì)胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標(biāo)本的人白細(xì)胞基因組dna ,并以此為模板成功擴(kuò)增出預(yù)期的109hpmbl目的基因片段。